20 QUESTIONS · 5 TOPICS · GEL ELECTROPHORESIS LAB · ABOUT 15 MINUTES

Insulin used to come from pig pancreases. Now bacteria make it.

In 1982 human insulin made by genetically modified E. coli became the first approved biotech drug. It started with enzymes that cut DNA at precise letters. Twenty questions from PCR to CRISPR, each explained, then cut some DNA and run it on a gel. No sign-up, nothing saved.

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THE BIOTECH TOOLKIT
1970Restriction enzymescut DNA at specific letters.
1973Recombinant DNAjoins genes from different species.
1983PCRcopies DNA a billion-fold in hours.
2003Human genomefully sequenced.
2012CRISPR-Cas9edits genes with a programmable guide.
QUESTION 1 / 20 · RECOMBINANT DNAMedium

Restriction enzymes are naturally used by bacteria to…

RESTRICTION DIGEST + GEL ELECTROPHORESIS

Cut a 10 kb piece of DNA and run it on a gel

Restriction enzymes cut DNA only at their recognition sequence. On a gel, an electric field pulls the negatively charged fragments towards the positive end, and small fragments travel furthest. Choose enzymes, then read the bands against the ladder.

CUT MAP · LINEAR 10 KB FRAGMENT
02.5k5k7.5k10k
−+
Ladder
Uncut
Digest
10 kb
8 kb
6 kb
5 kb
4 kb
3 kb
2 kb
1.5 kb
1 kb
500 bp
1.8 kb
2.8 kb
5.4 kb
FRAGMENTS3 fragments
5.4 kb2.8 kb1.8 kb
Total: 10,000 bp
A straight piece cut 2 times gives 3 fragments: one more than the number of cuts. Read each band's size by comparing it with the ladder on the left.

What this biotechnology test covers, in short

Twenty multiple-choice questions at upper-secondary to first-year university level, four on each of five topics: recombinant DNA, DNA tools and analysis, genome editing, medical biotechnology, and agricultural and industrial biotechnology.

It builds on the molecular biology and genetics tests. The DNA map in the lab is illustrative, but EcoRI, BamHI and HindIII and their sites are real. The test is our own and not normed.

By What's Your IQ · Reviewed 5 Oct 2026
Format
20 multiple-choice questions
Level
A-level / AP to first-year
Bonus
Restriction digest and gel simulator
Milestone
First recombinant DNA, 1973

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Reading a gel is visual deduction. How sharp is yours?

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